The effect of a period of interval training on the expression of G6Pase and PEPCK genes in the liver tissue of obese type 2 diabetic rats
محورهای موضوعی : تمرین ورزشی و بیماری ها
Asghar zolfaghari
1
,
َAbdolali Banaeifar
2
,
Sajad Arshadi
3
1 - PHD student,Department of Exercise Physiology, ST.C., Islamic Azad University, Tehran, Iran
2 - Associated Professor,Department of Exercise Physiology, ST.C., Islamic Azad University, Tehran, Iran.
3 - Associated Professor,Department of Exercise Physiology, ST.C., Islamic Azad University, Tehran, Iran.
کلید واژه: Interval training, Obese rats, Type 2 diabetes, Gene expression, G6Pase, PEPC,
چکیده مقاله :
Background: The present study was conducted to determine the effect of 10 weeks of interval training on the expression of G6Paseo PEPCK genes, which are related to gluconeogenesis in liver tissue in type 2 obese rats.
Materials and Methods: The statistical population of the present study consisted of obese type 2 diabetic rats. For this purpose, twenty-one 10-week-old Wistar rats were randomly divided into 3 groups (7 rats in each group): 1) healthy control 2) interval training 3) obese control group. At the beginning of the protocol, all rats were fed a high-fat diet for 8 weeks. Then, except for the obese control group, three other groups were made diabetic by intraperitoneal injection of STZ and then participated in a period of interval training.
48 hours after the last activity session, rats were anesthetized with a combination of xylazine and ketamine. After removing the tissue, the preparation steps were performed to examine the expression of the desired gene. The expression of the G6Pase and PEPCK genes was measured using the Realtime PCR method. Central and dispersion indices were used to describe the data statistically. The Shapiro-Wilk test was used to examine the data status and to examine the research hypotheses, the one-way analysis of variance (ANOVA) test was used and, if significant, the Tukey post hoc test was used to show the difference between the groups. The significance level was considered to be P≥0.05.
Results: The results of the present study showed that diabetes led to increased expression of G6Pase and PEPCK in the diabetic group compared to the healthy obese control group(p=.001). In addition, interval training was able to change these indices towards the values of the control group.
Conclusions: Based on the findings of the present study, improvement in the response of G6Pase and PEPCK to interval training as genes effective in the gluconeogenesis process leads to a decrease in the rate of gluconeogenesis and possibly an improvement in glycemic status in type 2 diabetic rats.
Background: The present study was conducted to determine the effect of 10 weeks of interval training on the expression of G6Paseo PEPCK genes, which are related to gluconeogenesis in liver tissue in type 2 obese rats.
Materials and Methods: The statistical population of the present study consisted of obese type 2 diabetic rats. For this purpose, twenty-one 10-week-old Wistar rats were randomly divided into 3 groups (7 rats in each group): 1) healthy control 2) interval training 3) obese control group. At the beginning of the protocol, all rats were fed a high-fat diet for 8 weeks. Then, except for the obese control group, three other groups were made diabetic by intraperitoneal injection of STZ and then participated in a period of interval training.
48 hours after the last activity session, rats were anesthetized with a combination of xylazine and ketamine. After removing the tissue, the preparation steps were performed to examine the expression of the desired gene. The expression of the G6Pase and PEPCK genes was measured using the Realtime PCR method. Central and dispersion indices were used to describe the data statistically. The Shapiro-Wilk test was used to examine the data status and to examine the research hypotheses, the one-way analysis of variance (ANOVA) test was used and, if significant, the Tukey post hoc test was used to show the difference between the groups. The significance level was considered to be P≥0.05.
Results: The results of the present study showed that diabetes led to increased expression of G6Pase and PEPCK in the diabetic group compared to the healthy obese control group(p=.001). In addition, interval training was able to change these indices towards the values of the control group.
Conclusions: Based on the findings of the present study, improvement in the response of G6Pase and PEPCK to interval training as genes effective in the gluconeogenesis process leads to a decrease in the rate of gluconeogenesis and possibly an improvement in glycemic status in type 2 diabetic rats.
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